Transcription of long hypothetical orfs in Trypanosoma cruzi: The epimastigote stage uses trans-splicing sites that generate short 5′ UTRs
نویسندگان
چکیده
منابع مشابه
Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms.
Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phosphoceramide subfractions, the latter accounting for 80-85% of the total IPL. The neutral lipids rele...
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A guanosine 5'-[gamma-[35S]thio]triphosphate-binding activity was detergent-extracted from Trypanosoma cruzi membranes. This binding activity was co-eluted from gel-filtration columns with a factor which, in a heterologous reconstitution system, blocks glucagon stimulation of adenylate cyclase activity in liver membranes. ADP-ribosylation of these membranes by pertussis toxin eliminated this bl...
متن کاملExperimental and Mathematical-Modeling Characterization of Trypanosoma cruzi Epimastigote Motility
The present work is aimed at characterizing the motility of parasite T. cruzi in its epimastigote form. To that end, we recorded the trajectories of two strains of this parasite (a wild-type strain and a stable transfected strain, which contains an ectopic copy of LYT1 gene and whose motility is known to be affected). We further extracted parasite trajectories from the recorded videos, and stat...
متن کاملThe short and the long of UTRs
One of the revelations of the post-genomics era has been that that much more of the genome is transcribed than was previously imagined, and that ncRNAs rival protein coding transcripts in genomic abundance. A pre-mRNA splices out much non-coding – though not necessarily nonregulatory – RNA sequence, yet the mature messenger RNA often still retains a significant non-protein-coding RNA sequence t...
متن کاملPrimary structure of the oligosaccharide chain of lipopeptidophosphoglycan of epimastigote forms of Trypanosoma cruzi.
The lipopeptidophosphoglycan of epimastigote forms of Trypanosoma cruzi is composed of a glycan linked through a non-N-acetylated glucosamine residue to an inositol phosphorylceramide. Using conventional analysis techniques, including 1H, 13C, and 31P NMR spectroscopy and negative ion fast atom bombardment mass spectroscopy, the structure of the carbohydrate-containing part of the molecule is d...
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ژورنال
عنوان ژورنال: Experimental Parasitology
سال: 2011
ISSN: 0014-4894
DOI: 10.1016/j.exppara.2011.07.004